Selective binding of 2'-F-c-di-GMP to Ct-E88 and Cb-E43, new class I riboswitches from Clostridium tetani and Clostridium botulinum respectively.

Immunogenic and Protective Potentials of Recombinant Receptor Binding Domain and a C-Terminal Fragment of Clostridium botulinum Neurotoxin Type E

Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...

Comparative Study of Three Vaccinal Strains of Clostridium tetani Including Harvard 52, G5 and 49205 from Standpoint of Six Essential Factors to Evaluate Their Toxigenesis for Use in Tetanus Vaccine Production

Tetanus is an important disease which created by tetanospasmin toxin of Clostridium tetani. In this study we surveyed six important factors including LF, KF, MLD, pH, OD and total protein assay of Harvard 52 (H52), G5 (HG5) and 49205 (H49205) strains of the bacterium to determine which of them were more suitable for use in vaccine production. The mentioned strains were seperatedly reactivated i...

Common mesophilic anaerobes, including Clostridium botulinum and Clostridium tetani, in 21 soil specimens.

A relatively rich medium was markedly superior to a dilute medium for the isolation of anaerobic bacteria from soil. The obligate anaerobes isolated from 21 soil samples were all clostridia and the counts ranged from 2.7 X 10-2 to 3.3 X 10-6 per g. The organisms most frequently isolated were Clostridium subterminate, C. sordelii, C. sporogenes, C. indolis, C. bifermentans, C. mangenoti, and C. ...

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Commentary: A bacterial global regulator forms a prion

The recently reported identification, within the transcription terminator Rho of Clostridium botulinum (Cb-Rho), of a prion-like domain functionally similar to that of a yeast prion-forming protein, along with the amyloidogenicity conferred by it on Cb-Rho, represent findings of extraordinary scientific relevance (Yuan and Hochschild, 2017). In this respect, another recent study has shown that ...